Erdogan said in an address to a business forum in Bishkek on Sunday during a visit to Kyrgyzstan "America behaves like wild wolves. Don't believe them."
He added that negotiations were underway between Turkey and Russia over using national currencies instead of the US dollar in bilateral trade ties.
The US has imposed punitive economic measures on both Ankara and Moscow.
"Using the dollar only damages us. We will not give up. We will be victorious," Erdogan told the meeting, attended by Kyrgyz and Turkish businessmen as well as government officials.
Washington and Ankara are entangled in a dispute over an American pastor standing trial in Turkey over terror and espionage charges.
The so-called American Pastor Andrew Brunson has been accused of espionage and having links with the outlawed Kurdistan Workers’ Party (PKK) and the Gulen Movement, which Turkey blames for the 2016 failed military coup.
Brunson, who has already spent nearly two years behind bars in Turkey, has denied the charges, calling them “shameful and disgusting.”
If found guilty, he will face up to 35 years in jail. His imprisonment and then house arrest have caused one of the deepest rows between Turkey and the US.